Brief introduction of the principle of human alpha-fetoprotein heterogeneous L3elisa kit - Database & Sql Blog Articles

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Human alpha-fetoprotein heterogeneous L3 (AFP-L3) detection kit , English name: Human AFP-L3 ELISA Kit, human alpha-fetoprotein heterogeneous L3 (AFP-L3) detection kit product commonly used alias: Fetin heterogeneous L3 (AFP-L3) elisa kit, human alpha-fetoprotein heterogeneous L3 (AFP-L3) elisa detection kit, kit specification: 48/96T

A brief introduction to the principle: Variants of AFP or molecular heterogenetic of AFP is a single-chain glycoprotein with a sugar content of about 4%. The AFP heterogeneity is named after the AFP band on the LCA affinity electrophoresis from L1, L2, and L3.

The principle of human alpha-fetoprotein heterogeneous L3 (AFP-L3) detection kit : the level of human alpha-fetoprotein heterogeneous L3 (AFP.L3) was determined by double antibody sandwich method. The microporous plate was coated with purified human alpha-fetoprotein heterogeneous L3 (AFP.L3) antibody to prepare a solid phase carrier. In the experiment, samples and standards were added to the microplate, and HRP-labeled AFP was added. The L3 antibody forms an antibody-antigen-enzyme-labeled antibody complex, and after repeated washing, the substrate TMB is added for color development. TMB is converted to blue under the catalysis of peroxidase, and the reaction is terminated with sulfuric acid to convert to yellow. The color depth is positively correlated with the AFP.L3 content in the sample. The absorbance (OD value) was measured at a wavelength of 450 nm using a microplate reader, and the concentration of AFP.L3 in the test sample was calculated according to a standard curve.

In the face of the different needs of the domestic enzyme-linked immunoassay industry, our company has launched research-based elisa kits and commercial ELISA kits, and in response to national energy-saving and emission-reduction requirements, we have alpha-fetoprotein heterogeneous L3 (AFP- L3) The test kits are all environmentally friendly packaging materials. At the same time, in order to better meet the needs of customers, we have launched an economical ELISA kit, which makes everyone's experiments more economical. The company is constantly striving to reduce the cost of ELISA reagent raw materials, so as to better The overall price of the ELISA kit is saved. I believe that in the near future, the price of the ELISA kit will be cheaper and the experimental effect will be more stable!
For more information on the Elisa kit, please visit the website: http://