Hengyuan share: What are the ELISA methods?

Our company is a high-tech biological company, which has long-term supply of various species of elisa kits. Now

ELISA has been widely used in scientific research.

ELISA

It can be used to determine antigens and can also be used to determine antibodies.

There are three essential reagents in this assay: (1) solid phase antigen or antibody, ie "immunosorbent"

(immunosorbent)

(2) enzyme-labeled antigen or antibody, called "enzyme conjugate", "conjugate"

(conjugate)

(3) Substrate for the enzymatic reaction. Depending on the source of the reagent and the condition of the specimen and the specific conditions of the assay, various types of assays can be devised.

Hengyuan share: What are the ELISA methods:

1.

indirect

ELISA

(

Indirect ELISA

)

:

Used to screen antibodies (specific antibodies against specific antigenic components). This method is a test

The most common method for determining antibodies.

2.

Sandwich

ELISA

(

Sandwich ELISA

)

:

It is used to detect the amount of antigen of interest. The advantage is to avoid specific antibodies

Direct marking, but with increased steps and measurement time.

3.

competition

ELISA

(

Competitive ELISA

)

:

Used to determine antigen specificity or when cross-reactive components are included in the specimen to be tested

A method used to increase the specificity of the experiment. According to the principle of competitive binding between the labeled antigen and the same unlabeled antigen to be tested, it is mainly used for the determination of small molecule antigens.

4. ABS-ELISA

law,

ABS

Avidin

(avidin

Biotin

(biotin)

system

(system)

Abbreviation. Avidin is a sugar

Protein

60000

, each molecule consists of

4

A subunit that binds to biotin.