Column DNA gel recovery kit operating instructions - Huaqiang Electronic Network

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Kaixin micro test

Test probe P100-M3

Products and features

This kit is a kit for recovering DN segments from an agarose gel. The kit can be used efficiently and professionally.

A DNA-binding silicone membrane and a unique sol system. The DN segment of 50 bp-40 Kb can be recovered from TAE, TBE and SuperBuffer gels with a recovery efficiency of up to 90%. The experimental process is as follows:

This product features:

1. Efficient, recycling efficiency up to 90%.

2. Fast, the whole process takes only ten minutes.

3. High purity, the DNA recovered in this kit can be directly used in various molecular biology such as digestion, ligation, PCR, etc.

experiment.

4. Widely versatile, this kit can recover single-stranded, double-stranded and circular DNA.

5. The price is cheaper and cheaper than most domestic products of the same kind.

6. Easy to store, the kit can be placed at room temperature for several months without affecting its quality.

Use and effect

In the agar gel block containing DN section or in the DNA reaction solution, add 3 times volume (about 0.3-0.5).

mL) universal sol solution, water soluble at 65 ° C for 5-10 minutes, transfer the solution to the centrifugal adsorption column, centrifuge half

Clock, add 500-600 μL of universal wash column and centrifuge for half a minute. Repeat the washing step once. Centrifuge for half a minute甩

Dry the remaining liquid. Place the spin column in a new centrifuge tube, add 30-50 μL of DNA eluate, and centrifuge for half a minute to obtain the sample in a centrifuge tube.

Answer
Q: Why is it not good to recover the DNA in TBE gel by silica gel membrane adsorption column method?
A: Because boric acid in TBE reacts with -OH on the surface of silica gel, these -OH are necessary for DNA adsorption. For a detailed explanation, please refer to the technical data----Silica-DNA binding principle and influencing factors.