Erythrocyte lysate is the easiest way to remove red blood cells by lysing red blood cells with lysate, which neither damages nucleated cells nor removes red blood cells. Lysis lysis is a relatively mild method of red blood cell removal. It is mainly used for the separation and purification of tissue cells dispersed by enzymatic digestion, the separation and purification of lymphocytes, and the removal of red blood cells in experiments such as tissue protein and nucleic acid extraction. The tissue cells obtained by lysis of red blood cell lysate do not contain red blood cells, and can be further used for primary culture, cell fusion, flow cytometry, separation and extraction of nucleic acids and proteins, and the like.
Cell lysates are generally enzyme-containing
,
Red blood cell-specific surface antigen on the surface of red blood cells,
When the lysate contains an enzyme that can attack specific red blood cell surface antigens, it will only cause red blood cell deformation.,
Biological channel expansion,
Swell,
Pyrolysis,
Or cause red blood cell degeneration.
Without attacking other cells.
In addition, red blood cells have their own electronegativity.,
Osmotic fragility(
Usually a breakthrough point for some non-enzymatic cell lysates) ,
Suspension stability,
These are the differences that distinguish them from other types of cells..
Erythrocyte lysate is the use of these characteristics to lyse red blood cells.
In addition, red blood cell lysate can not be reached100%
Accurate lysis of red blood cells,
Will always lead to the lysis of other kinds of cells more or less.
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