mOrange antibody - orange fluorescent protein bright fluorescence imaging - Database & Sql Blog Articles

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The number of cyan, green, and yellow spectral classes in fluorescent protein engineering is relatively large, and the orange fluorescent protein is currently only developed in the orange portion of the spectrum (from about 555 to 580 nm). Even so, all three existing orange fluorescent proteins, with isolated coral reef species, are likely to be very useful in a variety of imaging scenarios. The orange fluorescent protein mOrange is used as a bright fluorescent protein (fluorescence intensity is 1.46 times that of EGFP) and is often used as a labeling and reporter group. mOrange is a mutation from the mRFP protein, and its fluorescence characteristics are: Ex/Em = 548 nm / 562 nm.

Spectraviewer-graph

Figure.1 Fluorescence characteristics of mOrange

How to choose the right mOrange tag antibody?

When we choose mOrange antibody, we mainly need it according to our own needs, especially for experimental applications. In addition, the more types of applications after an antibody is detected, the greater the choice in use; while the monoclonal antibodies from mouse sources generally have better specificity, stability, and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl mOrange antibody (if the dilution ratio of WB is 1:5000, the final solution is equivalent to 500 ml), 1 ml of mOrange antibody with lower potency (if the dilution ratio of WB test is 1:200) The final use solution is equivalent to 200ml).

Tel Product Name and Description Item No. Product Description

Anti-mOrange Monoclonal Antibody (9A10)

A02260

Details

Why recommend the Abbkine brand of the orange fluorescent protein mOrange tag antibody (clone 9A10)?

At present, the mOrange tag antibodies available on the market are domestically packaged or originated. The domestically packaged mOrange tag antibodies have insufficient quality stability, and after being packaged, their titers are also lost. Some of the mOrange tag antibodies originating in domestic laboratories have good detection activity and are difficult to control in batch stability. However, only a small number of native antibodies in China are polyclonal antibodies. As for the imported brands that supply mOrange tag antibodies, there are not many imported brands, even Abcam, Sigma and other brands can not provide mOrange tag antibody products.

"MOrange tag antibody comprehensive comparison"

Brand Item Number Application Type Price/Specification Abbkine A02260 WB: (1:5000)

605/50ul

1800/200ul

6020/1ml

Abcam No No Sigma No No No No No No No No

Therefore, we recommend Abbkine to you.

Anti-mOrange Monoclonal Antibody (9A10)

,

The immunogen of Abbkine's mOrange antibody is a recombinant mOrange protein, which has been continuously screened and optimized to finally obtain the best cell line (9A10). As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing. This antibody can be used for WB.

A02260-wb

Antibody verification results, 1:5000 dilution

Tel Product Name and Description Item No. Product Description

Anti-mOrange MonoclonalAntibody(9A10)

A02260

Details

Abbkine also offers 1ml large packaging and larger custom-made mOrange antibodies, making it ideal for routine testing of mOrange immunoassays. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during use, please contact Abbkine local agent or Aimei Technology Co., Ltd. directly.

The Abbkine brand was established in California in 2012. With the rapid growth of the Asian market, Abbkine relocated its headquarters to China, transferred its production lines and related technologies, controlled by Chinese capital, and integrated various resources to historically open up China. Production base, based in China, serving the world. Combining the great advantages of Abbkine's US technology and Chinese manufacturing, we are committed to providing high quality, affordable immunoassay reagents, labels and internal reference antibodies, featured secondary antibodies, protein purification products and other molecular biology kits to meet Research and analytical needs of university research institutes, hospital research, and biomedical research institutions around the world.

Abbkine-logo As the general agent of Abbkine China, Amy Jiejie will provide customers with the most cost-effective routine immunological reagents, such as various tag antibodies, internal reference antibodies, conventional primary antibodies and a full range of secondary antibodies, antibody detection substrates, protein purification products and Related kits, etc. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line.

400-6800-868

Contact Amytech Co., Ltd. for product information and complete experimental solutions.

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